#PRESTAINED PROTEIN LADDER WESTERN BLOT VERIFICATION#

We did compare the migration patterns of SMOBIO’s Protein Markers with other brands, and we concluded that it was difficult to define “precision” due to the reasons mentioned above. For example, a protein which is highly hydrophilic might show a particular higher position in the SDS-PAGE analysis when compared to a hydrophobic one. It is known that the analysis of protein size by an SDS-PAGE is only for “estimation” because of the intrinsic variation of amino acid composition in all proteins including stained and non-stained ones. Usually, pre-stained marker is written on “estimated molecular weight” for caution. MOPS, and MES buffer) and after Western blotting to nitrocellulose membrane. Under suggested conditions, PM5200 ExcelBand™ 3-color Pre-Stained Protein Ladder Broad Range resolves 15 major bands in SDS-PAGE ( Tris-Glycine, Three reference bands - 75 kDa (red), 40 kDa (green), and 20 kDa (blue)Īpproximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate (pH 7.5 at 25☌), 2% SDS, 0.2 mM DTT, 3.6 M urea, and 15% (v/v) glycerol). Ready-to-use - Premixed with a loading buffer for direct loading, no need to boil.

#PRESTAINED PROTEIN LADDER WESTERN BLOT VERIFICATION#

The PM5200 3-color Pre-Stained Protein Ladder Broad Range is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. Proteins are covalently coupled with different chromophores for easy identification of bands, with three reference proteins carrying enhanced intensity corresponding to a blue band at 20 kDa, green at 40 kDa, and red at 75 kDa, respectively, as separated on SDS-PAGE (Tris-Glycine buffer). 5ul of protein ladder samples was loaded into each lane.The PM5200 3-color Pre-Stained Protein Ladder Broad Range is a ready-to-use three-color protein standard with 15 pre-stained proteins covering a wide range of molecular weights from 5 to 245 kDa in Tris-Glycine Buffer (3.5 to 235 kDa in Bis-Tris (MOPS) buffer and Bis-Tris (MES) buffer). *NuPAGE Bis-Tris 4-12% was used to run SDS-PAGE in MOPS and MES buffer. ► Enhanced transfer, wash, and stripping durabilities.Īpproximately 0.2~0.4 mg/ml of each protein in buffer ( 62.5 mM Tris-H3PO4 (pH 7.5 at 25 ☌), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN3, 33% glycerol.)ĥ μl of BLUltra Prestained Protein Ladder (PM001-0500) resolves 10 bands in 4-20% SDS-PAGE (Tris-glycine buffer) and after Western blotting to the nitrocellulose membrane. ► Clear separation map from 6.5 kDa to 270 kDa in the Tris-Glycine system. ► Better performance for higher molecular weight proteins. ► Higher Added Value: Tri-colors and 10 bands with premium quality. ► One-Vial-Fits-All: New broad-range protein ladder from 6.5kDa to 270kDa. That's why we're pleased to launch the color-enhanced BLUltra prestained protein standards.Īs you may see the comparison data shown below, BLUltra shows a better resolution than the BLUeye & BlueRAY for the protein bands from 95 kDa to 270 kDa. Nowadays, more and more researchers are studying the high molecular weight proteins, meaning they may need high-resolution prestained protein standards, especially with high molecular weights. Caution: Not intended for human or animal diagnostic or therapeutic uses. * supplement data should be considered for more accurate adjustment. Note: The apparent molecular weight of each protein (kDa) has been determined by calibration against an unstained protein ladder in each electrophoresis condition. Apply more for thicker (>1.5 mm) or larger gel.2~3 μl per well for general Western transferring.3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively.Migration patterns of BLUltra Prestained Protein Ladder in different electrophoresis conditions are listed below : Guide for Molecular Weight Estimation (kDa) The ladder is supplied in the gel loading buffer and is ready to use. The BLUltra Prestained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verifying Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The BLUltra Prestained Protein Ladder is a three-color protein standard with 10 pre-stained proteins covering a wide range of molecular weights from 6.5 to 270 kDa. Proteins are covalently coupled with a blue chromophore except for three reference bands (two orange bands at 30 kDa and 270 kDa and one green band at 52 kDa) when separated on SDS-PAGE (Tris-glycine buffer).

Prestained protein ladder western blot